The findings of my Master’s thesis highlighted the process of spontaneous differentiation of human embryonic stem cells and identified factors that can vastly influence the differentiation status of the cells. In addition, my novel approach of picking and reseeding single cells for colony formation and analyzing the cells, established a way to maintain the intactness of …

Abstract Human embryonic stem (hES) cells are capable of differentiating into a wide range of specialized cell types. Analysis of hES cells at the single colony or single cell level under cultured conditions is critical to understanding the molecular basis of hES cell differentiation. We have developed a new approach, in which single colonies of …